ABSTRACT
Objective: To determine the effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of developing femur of BALB/c mice
Study Design: Laboratory based randomized control trial
Place and Duration of Study: The study was conducted at anatomy department, Army Medical College [AMC], Rawalpindi, in collaboration with National Institute of Health [NIH], Islamabad from Oct 2014 to Oct 2015
Material and Methods: A total of thirty [30] BALB/c mice [15 male, 15 female], three weeks old and weighing 12-14 g, were taken and divided in to three equal groups of 10 mice each [5 male, 5 female]. The control group G1 was given normal diet with water ad libitum. In addition to the same diet, animals in experimental group G2 were given 10 mg of caffeine per 100 g body weight once a day on alternate days through oral gav age for 60 days. All animals of group G3 for 60 days were given caffeine 10mg/100gm body weight on alternate day and vitamin D3 0.1?g per day by oral gav age. At completion of the experiment, effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of mice's femur was analyzed
Results: The mean +/- SD of height of proliferative and hypertrophy zones of control group G1 animals was found to be 31.5 +/- 4.1?m and 43 +/- 2.6?m, respectively. The mean +/- SD of height of proliferative and hypertrophy zones of experimental group G2 animals was observed as 59 +/- 3.2?m and 72.5 +/- 7.2?m, respectively. The mean +/- SD of height of proliferative and hypertrophy zones of experimental group G3 animals was measured as 46 +/- 4.6?m and 54 +/- 4.6?m, respectively
Conclusion: Caffeine intake altered the height of proliferative and hypertrophy zones of the epiphyseal cartilage of developing femur; however treatment with vitamin D3 ameliorated this effect
ABSTRACT
Objective: To determine the effect of caffeine on the body weight of BALB/c mice
Study Design: Lab based randomized control trial
Place and Duration of Study: The study was conducted at Anatomy Department, Army Medical College [AMC], Rawalpindi, in collaboration with National Institute of Health [NIH], Islamabad, from Oct 2014 to Oct 2015
Material and Methods: Three weeks old BALB/c mice, twenty [20] in number [10 male, 10 female], weighing 12-14 g, were taken and divided into two groups with 10 mice [5 male, 5 female] in each group. The control group G1 was given standard diet with water ad libitum. In addition to the standard diet, the animals in experimental group G2 were given 10mg of caffeine per 100g body weight once a day on alternate days, three days in a week by oral gavage for 60 days. At the conclusion of the experiment, body weights of the mice in both the groups were measured to determine the influence of caffeine
Results: The mean final body weight of mice of control group G1 was observed as 31.2 +/- 1.932g while the mean final body weight of experimental group G2 was found to be 27.1 +/- 2.025g. As compared to control group G1, the final body weight of animals in experimental group G2 was found to be less
Conclusion: Ingestion of caffeine has negative effect on the accretion of body weight in mice
ABSTRACT
Objective: To determine the effects of caffeine ingestion on the development of femur and role of vitamin D[3] in preventing these effects in BALB/c mice
Study Design: Experimental study
Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi, in cooperation with NIH [National Institute of Health], Islamabad, from October 2014 to October 2015
Methodology: Thirty [100%] BALB/c mice, 50% male and female each, three weeks old, weighing 12-14 grams were taken and divided equally and randomly into three groups, each having 10 [33.3%] mice; 5 [16.6%] male and female. G[1] [control group] was given normal diet with water ad libitum. G[2] and G[3] [experimental groups] were given 10 mg of caffeine per 100g body weight, three days a week, through oral gavage for 60 days on alternate days. However, experimental group G[3] was additionally provided 0.1micro g vitamin D[3] daily, through oral gavage for 60 days. Experimental groups were compared with control group and data was analyzed statistically
Results: The mean weight of mice femur of G[1] [control group] was 0.387 +/-0.019 g; while mean weights of right femur of G[2] and G[3] [experimental groups] were 0.316 +/-0.020 g and 0.345 +/-0.020 g, respectively. Similarly, mean right femur length of group G[1] was 20.70 +/-0.609 mm; while for groups G[2] and G[3], it was 24.382 +/-1.087 mm and 22.966 +/-0.822 mm, respectively. In comparison with group G[1] for groups G[2] and G[3], femur weight decreased, however femur length increased
Conclusion: Caffeine intake caused femur length to increase and weight to decrease, but treatment with vitamin D[3] ameliorated these effects of caffeine